fig8

Figure 8. Chemogenetic inhibition of Aldh1a1⁺ nigrostriatal DANs does not affect food-seeking behavior. (A) Quantification of rewards earned by control mCherry [n = 9, 8 Male (M)/1 Female (F)] and hM4Di (n = 11, 5 M/6 F) AAV-injected TA double KI mice during a 2-hour free-feeding session on the FED3 device. Data are presented as mean ± SEM; unpaired t-test, P = 0.251; (B) Quantification of pellets earned in FR1 training on FED3 across three sessions. Two-way ANOVA with Tukey’s multiple comparison test: P = 0.3325 (session 1), 0.5549 (session 2), and 0.4928 (session 3); (C) Quantification of nose-poking accuracy in FR1 training on FED3 across three sessions. Two-way ANOVA with Tukey’s multiple comparison test: P = 0.5333 (session 1), 0.1080 (session 2), and 0.4305 (session 3). Aldh1a1⁺: Aldehyde dehydrogenase 1A1-positive; DANs: dopaminergic neurons; AAV: adeno-associated virus; TA: Th^Flp; Aldh1a1^CreERT2; KI: knock-in; FED3: Feeding Experimentation Device 3; SEM: standard error of the mean; FR1: fixed-ratio 1; ANOVA: analysis of variance.