fig7

Figure 7. Chemogenetic inhibition of Aldh1a1⁺ nigrostriatal DANs severely impairs rotarod motor skill learning. Rotarod motor skill learning in TA double KI mice injected with either control mCherry [n = 9, 8 Male (M)/1 Female (F)] or hM4Di AAVs (n = 11, 5 M/6 F) in the SNc. Mice underwent one training session per day for six consecutive days, with JHU37160 administered prior to each session. Data are presented as mean ± SEM. Two-way ANOVA, mCherry vs. hM4Di effect: F (1, 18) = 8.227, ^*P = 0.012. Aldh1a1⁺: Aldehyde dehydrogenase 1A1-positive; DANs: dopaminergic neurons; TA: Th^Flp; Aldh1a1^CreERT2; KI: knock-in; AAVs: adeno-associated viruses; SNc: substantia nigra pars compacta; SEM: standard error of the mean; ANOVA: analysis of variance.