fig6

Figure 6. Chemogenetic inhibition of Aldh1a1⁺ nigrostriatal DANs impairs spontaneous locomotion. (A-D) Quantification of total (A) (^***P = 0.0006), ambulatory (B) (^**P = 0.0013), rearing (C) (^**P = 0.0036), and fine (D) (^***P = 0.0002) movement in TA double KI mice injected with either control mCherry [n = 9, 8 Male (M)/1 Female (F)] or hM4Di AAVs (n = 11, 5 M/6 F) in the SNc. Locomotor activity was measured during a 30-minute open-field test following i.p. injection of JHU37160. Data are presented as mean ± SEM; unpaired t-test. Aldh1a1⁺: Aldehyde dehydrogenase 1A1-positive; DANs: dopaminergic neurons; TA: Th^Flp; Aldh1a1^CreERT2; KI: knock-in; AAVs: adeno-associated viruses; SNc: substantia nigra pars compacta; SEM: standard error of the mean.