fig4

Figure 4. Chemogenetic inhibition of Calb1⁺ nigrostriatal DANs affects food-seeking behavior. (A) Quantification of rewards earned by control mCherry [n = 8, 3 Male (M)/5 Female (F)] and hM4Di (n = 8, 4 M/4 F)-expressing TC double KI mice during a 2-hour free-feeding session on the FED3 device. Data are presented as mean ± SEM; unpaired t-test, P = 0.251; (B) Quantification of pellets earned in FR1 training on FED3 across three sessions. Two-way ANOVA with Tukey’s multiple comparison test: ^****P < 0.0001 (session 1), ^**P = 0.0086 (session 2), and 0.5036 (session 3); (C) Quantification of nose-poking accuracy in FR1 training on FED3 across three sessions. Two-way ANOVA with Tukey’s multiple comparison test: ^*P = 0.0495 (session 1), ^**P = 0.0096 (session 2), and 0.6679 (session 3). Calb1⁺: Calbindin 1-positive; DANs: dopaminergic neurons; TC: Th^Flp; Calb1^IRESTCre; KI: knock-in; FED3: Feeding Experimentation Device 3; SEM: standard error of the mean; FR1: fixed-ratio 1; ANOVA: analysis of variance.