fig3

Figure 3. Chemogenetic inhibition of Calb1⁺ nigrostriatal DANs severely impairs rotarod motor skill learning. Rotarod motor skill learning in TC double KI mice injected with either control mCherry [n = 10, 3 Male (M)/7 Female (F)] or hM4Di AAVs (n = 10, 4 M/6 F) in the SNc. Mice underwent one training session per day for six consecutive days, with JHU37160 administered prior to each session. Data are presented as mean ± SEM. Two-way ANOVA, mCherry vs. hM4Di effect: F (1, 18) = 22.2, ^***P = 0.0002. Calb1⁺: Calbindin 1-positive; DANs: dopaminergic neurons; TC: Th^Flp; Calb1^IRESTCre; KI: knock-in; AAVs: adeno-associated viruses; SNc: substantia nigra pars compacta; SEM: standard error of the mean; ANOVA: analysis of variance.