fig2

Figure 2. Chemogenetic inhibition of Calb1⁺ nigrostriatal DANs impairs spontaneous locomotion. (A-D) Quantification of total (A) (^***P = 0.0005), ambulatory (B) (^**P = 0.0016), rearing (C) (^**P = 0.0014), and fine (D) (^**P = 0.001) movement in TC double KI mice injected with either control mCherry [n = 10, 3 Male (M)/7 Female (F)] or hM4Di AAVs (n = 10, 4 M/6 F) in the SNc. Locomotor activity was measured during a 30-minute open-field test following i.p. injection of JHU37160. Data are presented as mean ± SEM; unpaired t-test; (E-H) Quantification of spontaneous locomotion: total (E) (P = 0.540), ambulatory (F) (P = 0.435), rearing (G) (P = 0.616), and fine (H) (P = 0.862) movement with the same cohort of mice as in (A-D) following saline injection. Data are presented as mean ± SEM; unpaired t-test. Calb1⁺: Calbindin 1-positive; DANs: dopaminergic neurons; TC: Th^Flp; Calb1^IRESTCre; KI: knock-in; AAVs: adeno-associated viruses; SNc: substantia nigra pars compacta; SEM: standard error of the mean.