fig1

Figure 1. Targeted expression of the chemogenetic inhibitor hM4Di in Calb1⁺ nigrostriatal DANs. (A) Schematic depicting the stereotactic delivery of control mCherry or hM4Di AAVs in the SNc of Th^Flp; Calb1^IRESCre (TC) double KI mice; (B) Representative images showing mCherry (red), ALDH1A1 (green), and TH (magenta) expression in the SNc of control mCherry (left panel) and hM4Di (right panel)-expressing mice. Scale bar: 1 mm; (C) Magnified images of the boxed areas in (B), with arrows indicating neurons co-expressing mCherry and ALDH1A1. Scale bar: 100 mm; (D) Representative images illustrating mCherry (red), ALDH1A1 (green), and TH (magenta) staining in the striatum of control mCherry-expressing mice. Scale bar: 1 mm. Calb1⁺: Calbindin 1-positive; DANs: dopaminergic neurons; AAVs: adeno-associated viruses; SNc: substantia nigra pars compacta; KI: knock-in; TH: Tyrosine Hydroxylase.