fig2

Figure 2. Movement disorders in P56S KI mice. (A-F) At 12 months of age, large cohorts of male WT and P56S KI mice (n ≥ 25) were assessed for the grip strength of the forelimbs and hindlimbs (A); the severity score in the hindlimb clasping test (B); the ambulatory movement, rearing movement, and center time in the open field test (C); the latency to fall in the rotarod test (D); the arm entries and percent alternations in the Y maze test (E); and the distance traveled, discrimination index, and novelty preference in the new object recognition test (F); (G-J) Pearson correlation analysis of the ambulatory movement in the open field test with the rearing movement in the open field test (G); the fall latency in the rotarod test (H); the arm entries in the Y maze test (I), and the distance traveled in the novel object recognition test (J). Unpaired t test: not significant (ns) P = 0.4879 (forelimbs) and ns P = 0.4347 (hindlimbs) in (A); ns P = 0.8912 in (B); ^****P < 0.0001 (ambulatory movement), ^*P = 0.0451 (rearing movement), and ns P = 0.0923 (center time) in (C); ^*P = 0.0107 in (D); ^**P = 0.0021 (arm entries) and ns P = 0.7743 (percent alternations) in (E); ^**P = 0.0013 (distance traveled), ns P = 0.5154 (discrimination index), and ns P = 0.5154 (novelty preference) in (F). KI: Knock-in; WT: wild-type.