fig5

Figure 5. Melatonin retards the iron deposition process in the brain of P301S mouse brain. (A) Perl’s DAB staining of the hippocampal CA1 region in P301S mice. Scale bar = 50 μm; (B-G) Western blotting analysis and quantification of DMT1, FPN, TFR, TF, and FTH with β-actin as an internal control. Values are represented as the means ± SEM (n = 8). ^*P < 0.05. DAB: 3,3,-Diaminobenzidine tetrahydrochloride; DMT1: divalent metal transporter 1; FPN: ferroportin; TFR: transferrin receptor; TF: transferrin; FTH: ferritin heavy chain; SEM: standard error of the mean.