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Figure 2. The characteristics of mtDNA modification in response to myocardial infarction. (A) Western blotting analysis for DNMT1 levels in mitochondria post-MI at different time points. (B) Detection of 5-mc levels in mtDNA in mitochondria post-MI at different time points. (C-E) Analysis of representative mRNA regulated by mtDNA in heart tissues post-MI. (F) Western blotting analysis of OXPHOS subunits in heart tissues post-MI at different time points. (G) Western blotting analysis of 4-HNE-conjugated protein levels in heart tissues at different time points post-MI. All results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test for multi-group comparisons. *P < 0.05, **P < 0.01, ***P < 0.001.