fig4

Figure 4. Myocardial lipid deposition and inflammatory immunohistochemistry (IHC) infiltration in fetal (D135) hearts from ewes receiving either control or overnutrition diet (150% NRC recommendation) for 135 days. (A) Representative oil red O staining depicting lipid deposition; (B) Pooled lipid deposition from oil red O staining; (C) Representative elastin staining depicting neutrophil infiltration (blue staining = DAPI staining for nucleus); (D) Pooled neutrophil infiltration status from elastin staining; (E) Representative CD68 staining depicting monocyte infiltration (blue staining = DAPI staining for nucleus); and (F) Pooled monocyte infiltration status from CD68 IHC staining. Mean ± SEM, *P < 0.05 between groups; n = 8 (D) or 5 (E and F) fetuses per group.