REFERENCES
1. Gandolfi S, Lupon E, Varin A, et al. Evolution of cell therapies derived from adipose tissue: historical perspectives, current development trends and future directions. Biol Direct. 2025;20:95.
2. Hasiba-Pappas S, Opriessnig E, Nischwitz SP, et al. Optimizing autologous fat grafting: a systematic review of enhancement strategies and graft survival. Aesthet Surg J. 2025;Epub ahead of print.
3. Choudhery MS, Mahmood R, Harris DT. Pre-enrichment of adipose tissue grafts: potential and limitations. In: Haider KH, Editor. Handbook of stem cell applications. Singapore: Springer Nature Singapore; 2023. pp. 1-43.
4. Firriolo JM, Condé-Green A, Pu LLQ. Fat grafting as regenerative surgery: a current review. Plast Reconstr Surg. 2022;150:1340e-7.
5. Varghese J, Mosahebi A. Historical overview of stem cell biology and fat grafting. Aesthet Surg J. 2017;37:S1-3.
6. Mazzola RF, Mazzola IC. The fascinating history of fat grafting. J Craniofac Surg. 2013;24:1069-71.
7. Urbonas T, Kievišas M, Petrikaitė V, et al. Assessing adipocyte viability and surgeons’ work efficiency by comparing different liposuction methods. Plast Reconstr Surg Glob Open. 2023;11:e5190.
9. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7:211-28.
10. Shen S, Huo H, Ren H, Shao Y. Comparative efficacy and safety of cell-assisted and conventional lipotransfer in facial filling: a systematic review and meta-analysis. Aesthetic Plast Surg. 2024;48:1444-56.
11. Kato H, Mineda K, Eto H, et al. Degeneration, regeneration, and cicatrization after fat grafting: dynamic total tissue remodeling during the first 3 months. Plast Reconstr Surg. 2014;133:303e-13.
12. Yang Z, Dong L, Jin S, Han X, Li F. Comparison of microfat, nanofat, and extracellular matrix/stromal vascular fraction gel for skin rejuvenation: basic animal research. Aesthet Surg J. 2023;43:NP573-86.
13. Louis F, Sowa Y, Irie S, et al. Injectable prevascularized mature adipose tissues (iPAT) to achieve long-term survival in soft tissue regeneration. Adv Healthc Mater. 2022;11:e2201440.
14. Jeong W, Son J, Choi J, et al. Clinically relevant and precisely printable live adipose tissue-based bio-ink for volumetric soft tissue reconstruction. Adv Healthc Mater. 2025;14:e2402680.
15. Kang Y, Yeo M, Derman ID, et al. Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction. Bioact Mater. 2024;33:114-28.
16. Feng J, Fu S, Luan J. Regenerated fat induced by a decellularized adipose matrix can survive long-term in vivo. Acta Biomater. 2025;191:233-43.
17. Xiang X, Jiang Z, Che D, et al. Application of free serratus anterior muscle-fascial composite tissue flap and facial lipofilling in repairing progressive hemifacial atrophy. Asian J Surg. 2024;47:973-81.
18. He L, Liu X, Khatter NJ, Yu X, Washington KM, Shu M. Treatment of progressive hemifacial atrophy by cartilage graft and free adipofascial flap combined with three-dimensional planning. Plast Reconstr Surg. 2024;153:679-88.
19. Miller KD, Nogueira L, Devasia T, et al. Cancer treatment and survivorship statistics, 2022. CA Cancer J Clin. 2022;72:409-36.
20. Piatkowski AA, Wederfoort JLM, Hommes JE, et al. ; Breast Reconstruction With External Preexpansion & Autologous Fat Transfer vs Standard Therapy (BREAST) Trial Investigators. Effect of total breast reconstruction with autologous fat transfer using an expansion device vs implants on quality of life among patients with breast cancer: a randomized clinical trial. JAMA Surg. 2023;158:456-64.
21. Kronowitz SJ, Mandujano CC, Liu J, et al. Lipofilling of the breast does not increase the risk of recurrence of breast cancer: a matched controlled study. Plast Reconstr Surg. 2016;137:385-93.
22. Giannakaki AG, Stylianaki A, Giannakaki MN, et al. Stem-cell-assisted lipotransfer and platelet-rich plasma in breast reconstruction: a literature review. Aesthetic Plast Surg. 2025;49:5764-73.
23. Moltaji S, Hoffbauer S, Brown MH. Large-volume fat grafting to the breast with external expansion assist. Aesthet Surg J. 2023;43:NP401-12.
24. Uhrbom M, Muhl L, Genové G, et al. Adipose stem cells are sexually dimorphic cells with dual roles as preadipocytes and resident fibroblasts. Nat Commun. 2024;15:7643.
25. Dai Q, Yu Q, Xiao R, Cai L. Early intervention with autologous fat grafting to reduce hypertrophic scar formation: a prelimilary study. Aesthetic Plast Surg. 2021;45:2409-16.
26. Yu Q, Dai Q, Huang Z, et al. Microfat exerts an anti-fibrotic effect on human hypertrophic scar via fetuin-A/ETV4 axis. J Transl Med. 2023;21:231.
27. Alfarafisa NM, Chou Y, Santika R, Riestiano BE, Soedjana H, Syamsunarno MRAA. Adipose-derived stem cell products and combination therapies for the treatment of pathological scars: a review of current preclinical and clinical studies. Clin Cosmet Investig Dermatol. 2025;18:1309-37.
28. Strong AL, Rohrich RJ, Tonnard PL, Vargo JD, Cederna PS. Technical precision with autologous fat grafting for facial rejuvenation: a review of the evolving science. Plast Reconstr Surg. 2024;153:360-77.
29. Tan PC, Wang YW, Xie Y, et al. Enhancing skin regeneration during expansion: a multicenter randomized controlled trial of stromal vascular fraction and fat grafting. Plast Reconstr Surg. 2026;157:360-71.
30. Añorve Borquez IR. A new technique for remodelling and increasing the diameter of the legs through lipotransfer and vertical subcutaneous fasciotomy of the posterior tibial aponeurosis. Aesthetic Plast Surg. 2021;45:1642-50.
31. Mendes FH, Viterbo F. Fragmented fat transfer for massive weight loss gluteal contouring. Plast Reconstr Surg. 2022;149:624-7.
32. Cortes W, Mather T, Logiudice J. Hip expansion with fat grafting combined with abdominoplasty: an evolution of body contouring technique. Plast Reconstr Surg Glob Open. 2023;11:11.
33. Troell RJ. Breast augmentation in body contouring using autologous stem cell-enriched fat grafting: fifteen-year clinical experience. Aesthet Surg J Open Forum. 2025;14:5607.
34. Ostberg JE, Ahmed Z, Saatchi K, et al. Adipose tissue-targeted delivery of rosiglitazone with iron oxide nanoparticles ameliorates insulin resistance in male mice. Obesity. 2026;34:1068-80.
35. Ibanez S, Giolito MV, Al-Siyabi S, et al. Acidosis forces fatty acid uptake and metabolism in cancer cells regardless of genotype. Adv Sci. 2025;12:e05436.
36. Alessandri G, Coccè V, Pastorino F, et al. Microfragmented human fat tissue is a natural scaffold for drug delivery: potential application in cancer chemotherapy. J Control Release. 2019;302:2-18.
37. Alessandri G, Pessina A, Paroni R, et al. Single-shot local injection of microfragmented fat tissue loaded with paclitaxel induces potent growth inhibition of hepatocellular carcinoma in nude mice. Cancers. 2021;13:5505.
38. Zeira O, Ghezzi E, Pettinari L, et al. Case report: microfragmented adipose tissue drug delivery in canine mesothelioma: a case report on safety, feasibility, and clinical findings. Front Vet Sci. 2020;7:585427.
39. Thomas S, Chen S, Sbitany H, et al. Autologous fat grafting as a novel antiestrogen vehicle for the treatment of breast cancer. Plast Reconstr Surg. 2017;140:537-44.
40. Andele PK, Palazzolo S, Corona G, et al. Human omental mature adipocytes used as paclitaxel reservoir for cell-based therapy in ovarian cancer. Adv Healthc Mater. 2024;13:e2304206.
41. Wen D, Wang J, Van Den Driessche G, et al. Adipocytes as anticancer drug delivery depot. Matter. 2019;1:1203-14.
42. Mangraviti A, Tzeng SY, Gullotti D, et al. Non-virally engineered human adipose mesenchymal stem cells produce BMP4, target brain tumors, and extend survival. Biomaterials. 2016;100:53-66.
43. Bo Y, Wang Y, Han J, et al. Primary adipocytes as targetable drug depot to prevent post-surgical cancer recurrence. Mater Today Bio. 2024;25:101020.
44. Brysgel TV, Hood ED, Milosavljevic A, et al. Decoupling physisorption from chemisorption in clickable lipid nanoparticles. ACS Nanosci Au. 2026;6:348-56.
45. Ziadlou R, Pandian GN, Hafner J, et al. Subcutaneous adipose tissue: implications in dermatological diseases and beyond. Allergy. 2024;79:3310-25.
46. Lescoat A, Huang S, Carreira PE, et al. ; EUSTAR collaborators. Cutaneous manifestations, clinical characteristics, and prognosis of patients with systemic sclerosis sine scleroderma: data from the international EUSTAR database. JAMA Dermatol. 2023;159:837-47.
47. Wang J, Cai J, Zhang Q, Wen J, Liao Y, Lu F. Fat transplantation induces dermal adipose regeneration and reverses skin fibrosis through dedifferentiation and redifferentiation of adipocytes. Stem Cell Res Ther. 2022;13:499.
48. Khanna D, Caldron P, Martin RW, et al. Adipose-derived regenerative cell transplantation for the treatment of hand dysfunction in systemic sclerosis: a randomized clinical trial. Arthritis Rheumatol. 2022;74:1399-408.
49. Liu J, Wang J, Zhang Q, Lu F, Cai J. Clinical, histologic, and transcriptomic evaluation of sequential fat grafting for morphea: a nonrandomized controlled trial. JAMA Dermatol. 2024;160:425-33.
50. Del Papa N, Sambataro D, Zaccara E, et al. SAT0446 autologous fat transplantation has a long term efficacy on scleroderma skin fibrosis: results from a controlled study versus hyaluronic acid filler. Ann Rheum Dis. 2015;74:822.
51. Wang L, Li T, Ma X, et al. Exosomes from human adipose-derived mesenchymal stem cells attenuate localized scleroderma fibrosis by the let-7a-5p/TGF-βR1/Smad axis. J Dermatol Sci. 2023;112:31-8.
52. Wang HC, Sun ET, Zhao RC, et al. Adipose-derived stem cells attenuate skin fibrosis and improve fat retention of a localized scleroderma mouse model. Plast Reconstr Surg. 2023;151:97-107.
53. Perrier A, Pugnet G, Chaput B, Gandolfi S. Hand surgical and injectable treatments in systemic sclerosis: a systematic review of published cases. J Scleroderma Relat Disord. 2025;10:212-23.
54. Almadori A, Griffin M, Hyun J, Hansen E, Denton CP, Butler PEM. Long-term outcome of fat grafting to treat facial systemic sclerosis: a prospective cohort study. Plast Reconstr Surg. 2025;156:611-21.
55. Liu X, Li Z, Wang HC, et al. Single-cell RNA sequencing identifies inherent abnormalities of adipose-derived stem cells from nonlesional sites of patients with localized scleroderma. Cell Mol Biol Lett. 2024;29:115.
56. Rigotti G, Marchi A, Galiè M, et al. Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells. Plast Reconstr Surg. 2007;119:1409-22.
57. Kenny EM, Egro FM, Ejaz A, Coleman SR, Greenberger JS, Rubin JP. Fat grafting in radiation-induced soft-tissue injury: a narrative review of the clinical evidence and implications for future studies. Plast Reconstr Surg. 2021;147:819-38.
58. Luan A, Duscher D, Whittam AJ, et al. Cell-assisted lipotransfer improves volume retention in irradiated recipient sites and rescues radiation-induced skin changes. Stem Cells. 2016;34:668-73.
59. Yu P, Yang X, Qi Z. Deferoxamine preconditioning of irradiated tissue improves perfusion and fat graft retention. Plast Reconstr Surg. 2018;142:977e.
60. Sivalingam V, Tamber K. Understanding the impact of life with vulval lichen sclerosus. Br J Dermatol. 2022;187:840.
61. Boero V, Brambilla M, Di Loreto E, et al. Fat grafting in vulvar lichen sclerosus: long term follow-up. J Low Genit Tract Dis. 2023;27:365-72.
62. Gutierrez-Ontalvilla P, Giner F, Vidal L, Iborra M. The effect of lipofilling and platelet-rich plasma on patients with moderate-severe vulvar lichen sclerosus who were non-responders to topical clobetasol propionate: a randomized pilot study. Aesthetic Plast Surg. 2022;46:2469-79.
63. Paganelli A, Contu L, Condorelli A, et al. Platelet-rich plasma (PRP) and adipose-derived stem cell (ADSC) therapy in the treatment of genital lichen sclerosus: a comprehensive review. Int J Mol Sci. 2023;24:16107.
64. Qi L, Zhang F, Wang K, et al. Advancements in skeletal muscle tissue engineering: strategies for repair and regeneration of skeletal muscle beyond self-repair. Regen Biomater. 2025;12:rbaf050.
65. Song Y, Wang N, Shi H, et al. Biomaterials combined with ADSCs for bone tissue engineering: current advances and applications. Regen Biomater. 2023;10:rbad083.
66. Lee J, Lee S, Huh SJ, Kang BJ, Shin H. Directed regeneration of osteochondral tissue by hierarchical assembly of spatially organized composite spheroids. Adv Sci. 2022;9:e2103525.
67. Sastourné-Arrey Q, Mathieu M, Contreras X, et al. Adipose tissue is a source of regenerative cells that augment the repair of skeletal muscle after injury. Nat Commun. 2023;14:80.
68. Mitsui R, Matsukawa M, Nakagawa K, et al. Efficient cell transplantation combining injectable hydrogels with control release of growth factors. Regen Ther. 2021;18:372-83.
69. Choi JS, Yoon HI, Lee KS, et al. Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration. J Control Release. 2016;222:107-15.
70. Liang W, Han M, Li G, et al. Perfusable adipose decellularized extracellular matrix biological scaffold co-recellularized with adipose-derived stem cells and L6 promotes functional skeletal muscle regeneration following volumetric muscle loss. Biomaterials. 2024;307:122529.
71. Sollie M, Toyserkani NM, Bille C, Thomsen JB, Sørensen JA. Autologous fat grafting as treatment of postmastectomy pain syndrome: a randomized controlled trial. Plast Reconstr Surg. 2022;149:295-305.
72. Sowa Y, Nakayama I, Toyohara Y, Higai S, Yoshimura K. Pain-relieving effects of autologous fat grafting in breast cancer surgery: a scoping review. Plast Reconstr Surg Glob Open. 2024;12:e5909.
73. Pietramaggiori G, Ricci F, L’Erario S, Bassetto F, Scherer S. Minimally invasive scar release by autologous adipose tissue transfer for post-traumatic neuropathic pain. Regen Ther. 2024;25:302-7.
74. Loder SJ, Kim JS, Moroni EA, Peter Rubin J, Spiess AM. QS4: fat grafting for the dysesthetic digit: a discussion of technique and pilot data. Plast Reconstr Surg Glob Open. 2021;9:18.
75. Amodeo G, Niada S, Moschetti G, et al. Secretome of human adipose-derived mesenchymal stem cell relieves pain and neuroinflammation independently of the route of administration in experimental osteoarthritis. Brain Behav Immun. 2021;94:29-40.
76. Gao SJ, Liu DQ, Li DY, et al. Adipocytokines: emerging therapeutic targets for pain management. Biomed Pharmacother. 2022;149:112813.
77. Rezzonico Jost T, Lozito A, Mangani D, et al. CD304 + adipose tissue-derived mesenchymal stem cell abundance in autologous fat grafts highly correlates with improvement of localized pain syndromes. Pain. 2024;165:811-9.
78. Rangel-Azevedo C, Santana-Oliveira DA, Miranda CS, Martins FF, Mandarim-de-Lacerda CA, Souza-Mello V. Progressive brown adipocyte dysfunction: Whitening and impaired nonshivering thermogenesis as long-term obesity complications. J Nutr Biochem. 2022;105:109002.
79. Liu X, Zheng Z, Zhu X, et al. Brown adipose tissue transplantation improves whole-body energy metabolism. Cell Res. 2013;23:851-4.
80. Zhang Y, Cai Y, Zhang H, et al. Brown adipose tissue transplantation ameliorates diabetic nephropathy through the miR-30b pathway by targeting Runx1. Metabolism. 2021;125:154916.
81. Karanfil AS, Louis F, Matsusaki M. Brown adipose tissue engineering: advances, challenges, and future directions. Trends Biotechnol. 2026;44:957-74.
82. Wang O, Han L, Lin H, et al. Fabricating 3-dimensional human brown adipose microtissues for transplantation studies. Bioact Mater. 2023;22:518-34.
84. Pang YF, Shu L, Xia CW. Retrospective comparative study of different surgical methods for gastric ulcer perforation: Efficacy and postoperative complications. World J Gastrointest Surg. 2025;17:101896.
85. Hourneaux de Moura DT, Bestetti AM, Fernandes TR, Hojaij FC, Hourneaux de Moura EG. Autologous abdominal fat transplantation for treating complex GI fistulas: a promising new approach. Gastrointest Endosc. 2025;101:672-4.
86. Sadiq KO, Dobke MK, Boland BS, Lopez N, Eisenstein S. Autologous fat grafting for perianal fistula in Behçet’s disease: a case report. Inflamm Bowel Dis. 2025;31:601-2.
87. Dige A, Hougaard HT, Agnholt J, et al. Efficacy of injection of freshly collected autologous adipose tissue into perianal fistulas in patients with Crohn’s disease. Gastroenterology. 2019;156:2208-16.e1.
88. Aldakhil M, Albarrak RI, Alomar JA, Alnasr LI, Alassaf RM, Alhumaid NK. The efficacy of adipose-derived stem cell therapy for complex perianal fistulas in Crohn’s disease patients: a systematic review. Surg Open Sci. 2026;29:22-8.
89. Park MY, Yoon YS, Lee JL, et al. Comparative perianal fistula closure rates following autologous adipose tissue-derived stem cell transplantation or treatment with anti-tumor necrosis factor agents after seton placement in patients with Crohn’s disease: a retrospective observational study. Stem Cell Res Ther. 2021;12:401.
90. Kim SJ, Kim MJ, Lee YJ, et al. Innovative method of alopecia treatment by autologous adipose-derived SVF. Stem Cell Res Ther. 2021;12:486.
91. Nilforoushzadeh MA, Heidari-Kharaji M, Baiat Tork B, et al. The first study on autologous adipose tissue transfer in the treatment of lichen planopilaris. J Cosmet Dermatol. 2022;21:5295-7.
92. Evin N, Guray Evin S. Camouflage of postburn scarring alopecia using nanofat grafting and follicular unit hair transplantation. Plast Reconstr Surg. 2024;154:207-15.
93. Sadeghi S, Ghane Y, Hajizadeh N, Goodarzi A. Autologous adipose tissue injection in the treatment of alopecia: a mini-review. J Cosmet Dermatol. 2024;23:758-65.
94. Brown S, Dayan JH, Kataru RP, Mehrara BJ. The vicious circle of stasis, inflammation, and fibrosis in lymphedema. Plast Reconstr Surg. 2023;151:330e-41.
95. Tashiro K, Yoshioka Y, Ochiya T. Extracellular vesicles from adipose-derived stem cells relieve extremity lymphedema in mouse models. Plast Reconstr Surg. 2023;152:1011-21.
96. Frueh FS, Gassert L, Scheuer C, et al. Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model. J Tissue Eng. 2022;13:20417314221109957.
97. Jørgensen MG, Jensen CH, Hermann AP, et al. No clinical efficacy of adipose-derived regenerative cells and lipotransfer in breast cancer-related lymphedema: a double-blind placebo-controlled phase II trial. Plast Reconstr Surg. 2024;154:1172-82.
98. Dai T, Jiang Z, Cui C, et al. The roles of podoplanin-positive/podoplanin-negative cells from adipose-derived stem cells in lymphatic regeneration. Plast Reconstr Surg. 2020;145:420-31.
99. Hu L, Zhang N, Zhao C, Pan J. Engineering ADSCs by manipulating YAP for lymphedema treatment in a mouse tail model. Exp Biol Med. 2024;249:10295.
100. Pruttikul P, Sutthiwongkit T, Kunakornsawat S, Paiboonsirijit S, Pongpirul K. Enhanced technique of dural closure using autologous fat graft and Gelfoam for effective management of dural tear following interlaminar endoscopic lumbar spine surgery. Eur Spine J. 2024;33:2886-91.
101. Luzzi S, Doğruel Y, Güngör A, et al. Clival dural reconstruction via transnasal approaches: fat graft fixation technique. Neurosurg Focus. 2025;58:E4.
102. Diaz AR, Reina CO, Plaza G, Posadas ER, Arevalo FV, Iriarte MTG. Long-term follow-up after fat graft myringoplasty: do size and location matter? Ear Nose Throat J 2021;100:229S-34.
103. Chen AW, Chen CH, Lin TM, Chang AC, Tsai TP, Chang SY. Office-based structural autologous fat injection laryngoplasty for unilateral vocal fold paralysis. J Clin Med. 2022;11:4806.
104. Chang WD, Chen SH, Tsai MH, Tsou YA. Autologous fat injection laryngoplasty for unilateral vocal fold paralysis. J Clin Med. 2021;10:5034.
105. Haddad R, Ismail S, Khalaf MG, Matar N. Lipoinjection for unilateral vocal fold paralysis treatment: a systematic review and meta-analysis. Laryngoscope. 2022;132:1630-40.
106. Roychoudhury A, Yadav P, Alagarsamy R, Bhutia O, Goswami D. Outcome of stock total joint replacement with fat grafting in adult temporomandibular joint ankylosis patients. J Oral Maxillofac Surg. 2021;79:75-87.
107. Kilinskaite G, Kilinskaite N, Leketas M. The effectiveness of using autologous fat in temporomandibular joint ankylosis treatment with interposition arthroplasty method: a systematic literature review. Healthcare. 2025;13:2241.
108. Adelino Recasens R, Galvez-monton C, Martinez-falguera D, et al. Cardiac reparative therapy with adipose graft transposition procedure (AGTP) improves electrophysiological remodeling of chronic myocardial infarction. Eur Heart J. 2021;42:ehab724.3258.
109. Yao L, Wang Q, Zhang R, et al. Brown adipose transplantation improves polycystic ovary syndrome-involved metabolome remodeling. Front Endocrinol. 2021;12:747944.
110. Ye R, Yan C, Zhou H, et al. Brown adipose tissue activation by cold treatment ameliorates polycystic ovary syndrome in rat. Front Endocrinol. 2021;12:744628.
111. Yuan X, Hu T, Zhao H, et al. Brown adipose tissue transplantation ameliorates polycystic ovary syndrome. Proc Natl Acad Sci U S A. 2016;113:2708-13.
112. Sundaram VK, Schütza V, Schröter NH, et al. Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration. Cell Metab. 2023;35:2136-52.e9.
113. Saffari TM, Saffari S, Vyas KS, Mardini S, Shin AY. Role of adipose tissue grafting and adipose-derived stem cells in peripheral nerve surgery. Neural Regen Res. 2022;17:2179-84.
114. Sun Y, Chi X, Meng H, et al. Polylysine-decorated macroporous microcarriers laden with adipose-derived stem cells promote nerve regeneration in vivo. Bioact Mater. 2021;6:3987-98.
