fig1

Figure 1. Chronic β-FNA dose-dependently inhibits CXCL10 expression in NHA. Cells were initially cultured in growth medium containing 0.04-10 µmol/L β-FNA for 24 h; the medium was then replaced with serum-free medium containing β-FNA for an additional 48 h. IL-1β (3 ng/mL) was added to cultures for the final 24 h. CXCL10 in the medium was measured by ELISA. Data are presented as mean ± SEM (n = 8-9). CXCL10: interferon-γ inducible protein-10; NHA: normal human astrocytes; ELISA: enzyme-linked immunoabsorbant assay; SEM: standard error of the mean; β-FNA: beta-funaltrexamine; IL-1β: interleukin-1β