fig5

Figure 5. Loss of Phf15 affects the expression of genes involved in viral response and regulation of inflammatory processes in the absence of immune stimulation. A: Volcano plot representing the RNA-seq results. Orange dots represent differentially expressed genes in Phf15 knockout microglia compared to control (upregulated genes at a cutoff of log₂-fold change > 1.5 and P adj < 0.01; downregulated genes at a cutoff of log₂-fold change < -1.5 and P adj < 0.05); B: GO analysis for significantly upregulated genes showing biological process categories related to “response to virus” and “inflammatory response”; C: Upregulated genes associated with “response to virus” and “inflammatory response” in the no stimulation (baseline) condition. Relative FPKM values were obtained by normalizing FPKM values of Phf15 knockout SIM-A9 microglia to control FPKM values for each gene (n = 3 per condition). Statistics are by DESeq2: ^#P < 0.0001; D: Top 5 enriched transcription factor binding motifs for the set of upregulated genes in the no stimulation (baseline) condition. KO: knockout; FPKM: Fragments per kilobase million; GO: Gene ontology