fig4

Figure 4. Phf15 overexpression decreases the microglial inflammatory response. (A) Fold OE of Phf15 in SIM-A9 microglia (red bar) versus control cells (Ctrl, open bar). Data are mean ± SEM (n = 3 per condition). Unpaired t-test between Phf15 OE and control cells. Twenty-four-hour time course experiments showing relative mRNA expression levels of Tnfα (B), IL-1β (D), and Nos2 (F) after LPS stimulation. Baseline (0-h time point, no stimulation) expressions of Tnfα (C), IL-1β (E), and Nos2 (G) are displayed separately from time course experiments. Data are mean ± SEM (n = 3 per condition). Unpaired t-tests with Holm-Sidak correction for multiple comparisons between Phf15 OE and control cells within timepoint: *P < 0.05, **P < 0.01. OE: overexpression; LPS: lipopolysaccharide; Tnfα: tumor necrosis factor alpha; inducible; IL-1β: interleukin 1 beta; Nos2: nitric oxide synthase, inducible