fig2

Figure 2. Knockdown of Phf15 increases the magnitude of the microglial inflammatory response. (A) Knockdown efficiency for anti-Phf15 shRNAs shPhf15-1 (blue bar, 52% knockdown) and shPhf15-2 (red bar, 60% knockdown). Data are mean ± SEM (n = 3 per condition). One-way ANOVA with Dunnett’s multiple comparisons between shPhf15-1 or shPhf15-2 and shCtrl cells: **P < 0.01. Twenty-four-hour time course experiments showing relative mRNA expression levels of Tnfα (B) and Nos2 (D) after LPS stimulation of shRNAs shPhf15-1 and shPhf15-2 compared to shCtrl (control scrambled shRNA). No stimulation (0-h time point) is shown for Tnfα (C) and Nos2 (E). Data are mean ± SEM (n = 3 per condition). One-way ANOVA with Dunnett’s multiple comparisons for shPhf15-1 or shPhf15-2 relative to shCtrl cells for individual timepoints: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. LPS: lipopolysaccharide; Tnfα: tumor necrosis factor alpha; Nos2: nitric oxide synthase, inducible