fig5

Figure 5. Interleukin-1β (IL-1β)-induced p38 MAPK activation in C20 human microglial cells. C20 cells were exposed to media alone (unstimulated; US) or media containing IL-1β (20 ng/mL) for 10-60 min. Western blot analysis was used to measure levels of p-p38 mitogen-activated protein kinase (p38 MAPK), p38 MAPK, and β-tubulin in cytoplasmic protein extracts. The blots presented are representative of independent experiments (n = 5) and the data represent mean ± SEM. Bars with different letters are significantly different (P < 0.05) as determined by one-way ANOVA and Tukey’s pairwise comparisons