fig4

Figure 4. Interleukin-1β (IL-1β)-induced nuclear factor (NF)-κB p65 activation in C20 human microglial cells. C20 cells were exposed to media alone (unstimulated; US) or media containing IL-1β (20 ng/mL) for 10-60 min. Western blot analysis was used to measure levels of p-NF-κB p65, NF-κB p65, and β-tubulin in nuclear protein extracts. The blots presented are representative of independent experiments (n = 4-5) and the data represent mean ± SEM. Bars with different letters are significantly different (P < 0.05) as determined by one-way ANOVA and Tukey’s pairwise comparisons