fig3

Figure 3. Interleukin-1β (IL-1β)-induced inhibitory kappa B alpha (IκBα) activation in C20 human microglial cells. C20 cells were exposed to media alone (unstimulated; US) or media containing IL-1β (20 ng/mL) for 10-60 min. Western blot analysis was used to measure levels of p-IκBα, IκBα, and β-tubulin in cytoplasmic protein extracts. The blots presented are representative of independent experiments (n = 4-5) and the data represent mean ± SEM. Bars with different letters are significantly different (P < 0.05) as determined by one-way ANOVA and Tukey’s pairwise comparisons