fig2

Figure 2. Cytokine/chemokine expression in C20 human microglial cells. C20 cells were exposed to media alone (unstimulated; Unstim.) or media containing either lipopolysaccharide (LPS) (1 µg/mL) or interleukin-1β (IL-1β) (20 ng/mL) for 24 h. CXCL10, CCL2, and IL-6 levels in the culture medium were determined by ELISA and normalized to total cellular protein (as determined by the bicinchoninic acid method). Data represent mean ± SEM (n = 4-7). Bars with different letters are significantly different (P < 0.01) as determined by one-way ANOVA and Tukey’s pairwise comparisons