fig1

Figure 1. C20 human microglial cells express the microglial marker transmembrane protein 119 (TMEM119). C20 cells were exposed to media alone (unstimulated; US) or media containing interleukin-1β (IL-1β) (20 ng/mL) for 24 h. Top panel: western blot analysis was used to measure levels of TMEM119 and glial fibrillary acidic protein (GFAP) in whole cell lysates and β-tubulin was assessed as a loading control. Whole cell lysates from unstimulated and IL-1β (3 ng/mL)-stimulated normal human astrocytes (NHA) were used for comparison. The blots presented are representative of independent experiments (n = 3 for C20; and n = 2 for NHA). Bottom panel: fluorescent immunocytochemistry was used to further assess TMEM119 expression (green) in US (A-D) and IL-1β-stimulated (E-H) C20 cells; and nuclei were labeled with 4ʹ,6-diamidino-2-phenylindole (DAPI, blue). Images are shown at 400 × magnification. The arrows in box H highlight the cytoplasmic extensions