fig7

Figure 7. Quantification of phagocytosis using a colorimetric NBT assay. ROS generation by cells from different age groups in normal conditions was measured by using the colorimetric NBT assay done in triplicate set. Data are statistically interpreted by using one-way ANOVA followed by Tukey-Kramer test used for post-hoc multiple comparisons where P < 0.05 is considered significant. Significant difference associated with phagocytic ability present between different age groups where P value is less than 0.05 indicated by * symbol in graph. Graphical representation shows there is a significant increase of phagocytic ability in both late embryo and neonates as compared to the early embryo (indicated by * mark) whereas in case of adult the variation is insignificant with early embryo. Significant difference in phagocytic potential between these two groups (late embryo and neonates) with the adult is also indicated by * symbol