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Water Emerg Contam Nanoplastics 2022;1:8. 10.20517/wecn.2022.09 © The Author(s) 2022.
Open Access Editorial

How microplastics and nanoplastics shape antibiotic resistance?

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.

Correspondence to: Dr. Jie Wang, Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, 17 Qinghua E Rd, Haidian Qu, Beijing 100193, China. E-mail: jiewangcau@cau.edu.cn ; wangjie321@gmail.com

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    Academic Editor: Antonio Ginebreda | Copy Editor: Tiantian Shi | Production Editor: Tiantian Shi

    © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

    Despite the remarkable benefits and convenience of plastics to human lives, there is increasing awareness about the negative environmental impacts arising from the vast amount of plastic waste. Due to the environmental weathering over time, fragmentation of plastics can happen, leading to microplastic (< 5 mm) and nanoplastic (< 100 nm) generation. As a hot environmental topic in the past decade, more and more studies have focused on the subjects of microplastic and nanoplastic pollution, i.e., qualitative and quantitative methods, environmental fate and behavior, biological consequences, and potential removal techniques[1-5]. In addition, concerns about the relationship between antibiotic resistance and micro/nanoplastics have arisen with the recent one-health concept[6].

    Antibiotic resistance is one of the biggest threats to global health, as the proliferation of resistance reduces or eliminates the utility of antibiotics, rendering some clinical infections dangerously untreatable. Therefore, gaining insight into mechanisms driving the dissemination and propagation of antibiotic resistance genes (ARGs) is essential. The use of antibiotics is usually considered to be the main driving factor for the development and selection of ARGs[7]. Additionally, due to the co-selection mechanisms, anthropogenic levels of toxic heavy metals have also been associated with the dissemination of ARGs[8]. Recent evidence that microorganisms colonizing microplastics are resistant to antibiotics and that microplastics increase the gene exchange in aquatic ecosystems suggests that microplastics could have ramifications on disease transmission and treatment [Table 1]. To further illustrate the relationship between micro/nanoplastics and ARGs, we used the Web of Knowledge database to retrieve publications. The results were further viewed by CiteSpace[9]. The high-frequency keywords included microbial community disturbance, conjugative antibiotic resistant plasmid, occurrence removal, microplastic biofilm, and potential carrier [Figure 1], suggesting that researchers dominantly focused on the correlation between ARGs and microbial communities on plastics, the plastic-induced gene transfer, and vector roles of plastics.

    Figure 1. The co-occurrence of keywords and citation related to micro/nano-plastics and ARGs.

    Table 1

    The studies related to micro/nano-plastics and ARGs

    MP typeDesignMethods Main results Reference
    Plastic particlesFiled-collected samplesMetagenomics• Higher levels of ARGs were observed on plastics, and a correlation between ARGs and bacterial communities was observed[10]
    HDPEIn situ incubationHT-qPCR
    • A total of 82 ARGs, 12 MGEs, and 63 bacterial pathogens were detected in the plastisphere, and the correlation between ARGs and bacterial communities was observed[21]
    PE, PPEx situ microcosm with landfill leachateqPCR• MPs exhibited selective enrichment for ARGs in a ratio of 5.7-103 folds
    • Closer and more stable ARGs-bacterial taxa relations on MP surface affected the ARG transmission
    [22]
    PS, PP, PE, PET, PVCField-collected samplesqPCR• ARG absolute abundance showed: biofilms on plastics > sediment > water
    • Firmicutes showed significant correlations with sul1 and tetA on PE
    [23]
    PE, PPField-collected samplesqPCR• MPs selectively enriched antibiotic resistant genes with distinct ARG profiles, and a correlation between ARG profile and bacterial composition was obtained[13]
    PEEx situ microcosm with water samples qPCR• MPs can enrich antibiotics, ARGs and microbes from the surrounding water[12]
    PVCSewage microcosmHT-qPCR• PVC MPs could enrich bacteria and resistance genes from surrounding sewage[24]
    PE, PVCSewage microcosmHT-qPCR• MPs led to the increase of the total copies of ARGs and mobile genetic elements (MGEs) in the sewage
    • Multidrug ARGs and MGEs were enriched on plastisphere
    [25]
    PHA, PETIn situ incubationMetagenomics• Plastic type significantly influenced the ARG composition in the biofilms, and the abundance of multidrug resistance genes on PET was relatively higher
    • A good-fit correlation between ARG profile and bacterial composition was obtained
    [11]
    Aged PS MPsIncubation with goldfish Metagenomic• Microplastics promote the occurrence of ARGs in the intestine[26]
    PE, PP, PS, recycled PEEx situ microcosm with wastewaterqPCR• PP have the highest adsorption capacity of ARGs among all microplastics
    • Exposure to microplastics enhances horizontal gene transfer of ARGs
    [15]
    PS N/MPsEx situ microcosm with landfill leachateqPCR• ARGs were enriched more in the 200–500 nm MP group
    • The potential ARG-carrying bacteria increased in the N/MP exposed groups
    [19]
    PS N/MPSIncubation with earthwormsMetagenomics• Microplastics (MPs) impact the occurrence of ARGs in earthworm guts[27]
    PA, PET, PLAIndoor collected MPsqPCR• Relative abundance of ARGs on MPs was higher, and a correlation between ARGs and bacterial communities was observed[16]
    PS NPsIncubation with Enchytraeus crypticusHT-PCR• NPs enhanced the toxicity of tetracycline and promoted ARGs enrichment
    • NPs + TC caused more serious ARGs enrichment in E. crypticus than in soils
    [28]
    PS N/MPsE. coli incubation• PS particles with sizes ≤ 100 nm impacted the transformation of ARGs
    • PS particles interfered with plasmid replication inside E. coli, thus decreasing the bacterial transformation and affecting the dissemination of ARGs
    [20]
    PEEx situ incubationqPCR• PE MPs significantly increased the fold changes in the abundances of tetC, tetG, and tetW
    • Free ammonia contributed the most to the changes in host bacteria, which probably changed the fate of ARGs
    [29]
    PVCIn situ incubationqPCR• PVC MPs benefited the propagation of intI1 and tetE in the AGS system
    • PVC MPs positively correlated with some Accumulibacter clades and ARGs
    [30]
    PS NPsIn situ incubation qPCR• The thermal hydrolysis process reduced ARG propagation that can be encouraged by PS NPs[31]
    PETIncubation with mice HT-qPCR• MPs could exacerbate the effects of SMX on gut microbiota and ARG profile
    • SMX and MPs exposure significantly increased the relative abundances of ARGs
    [32]
    PE, PP, PS, PET, PCLIn situ incubationqPCR• Sociometric and environmental factors were the main drivers shaping the distribution characteristics of ARGs and MGEs
    • PP and HDPE particles are preferred substrates for obtaining diffuse ARGs
    [33]

    Microplastics could selectively enrich ARGs. By analyzing the metagenomic data of plastic particles from the North Pacific Gyre, Yang et al. (2019) first reported that the abundance of total ARGs on microplastics was significantly greater than that in seawater[10]. Since this study, understanding the roles of microplastics in carrying ARGs has become an important area to explore. The ARGs on the microplastics collected from the oceans[11], estuaries[12], urban rivers[13], farmlands[14], wastewater treatment plants[15], and even indoor air[16] have been estimated. Most studies illustrated that the ARG abundance and diversity were significantly different from those in the surrounding environments, which was closely associated with distinct microbial communities on the microplastics. Additionally, the bacterial communities on microplastics may be denser, further enhancing the horizontal gene transfer rate of ARGs on microplastics. Several studies have measured the indicator gene of horizontal gene transfer on microplastics and proved this hypothesis[13].

    Microplastics may also affect the ARGs in surrounding environments. For instance, Song et al. (2022) reported that biodegradable microplastics significantly changed the ARG compositions and broadened their bacterial hosts, which was due to the fact that generated water-soluble low molecular weight oligomers from the biodegradable microplastics may act as additional carbon sources for microbial assimilation[17]. Lu and Chen (2022) also found that the ARG characteristics in soils were changed after microplastic amendment, which was attributed to the sorbed phthalates[18]. Unfortunately, not enough studies have evaluated this question. Based on their natural properties, e.g., undissolved solid particles, it is not easy for microplastics to enter into bacteria and induce the expression of ARGs. Thus, the co-selection mechanisms may not be appropriate for microplastic exposure. They may not directly target the ARGs but indirectly through changing the communities of ARG bacterial hosts or leaching chemicals to show selective pressures.

    Although microplastics and nanoplastics share similar composition and origin, the nanospecific properties (e.g., transport properties, bioavailability, interactions with natural colloids, and potential toxicity) distinguish nanoplastics from microplastics. Therefore, nanoplastics may affect the dissemination of ARGs via different mechanisms. Shi et al. (2020) found that the ARGs in municipal landfill leachate were enriched in the nanoplastic exposed group. They reported that nanoplastics could induce the production of reactive oxygen species (ROS), which potentially increase bacterial membrane permeability and thus facilitate the intra-bacterial community transfer of mobile genetic elements[19]. Similarly, Hu et al. (2022) observed that nanoplastics can promote the transformation of ARGs, and the enhancing mechanism was the direct interaction of nanoplastics with membrane lipids and the indirect effect associated with bacterial oxidative stress response[20]. Both processes can induce the formation of pores on the cell membrane and increase the membrane permeability, thus enhancing gene transformation.

    Despite the growing literature on the effects of micro/nanoplastics on ARGs, most studies only observed the correlation between microbial communities and ARG profiles, which is not the underlying reason plastics could selectively recruit ARGs. For microplastics, we may hypothesize that the microplastics can selectively recruit microorganisms growing on microplastics, which contributes to the distinct ARG profiles on microplastics. The closer physical distance of microorganisms would induce the horizontal gene transfer. Thus, further studies should focus on why the microplastics have the ability to show selective recruitment and whether the levels of gene exchanges on microplastics are higher. For nanoplastics, their attack on the cell wall may be more important for gene exchanges. More attention should focus on how micro/nanoplastics mediate the ARG transformation.

    DECLARATIONS

    Authors’ contributions

    Made a substantial contribution to data curation, methodology, writing - original draft: Sun Y

    Made a substantial contribution to methodology, writing - reviewing and editing, supervision: Wang J

    Availability of data and materials

    Not applicable.

    Financial support and sponsorship

    None.

    Conflicts of interest

    Both authors declared that there are no conflicts of interest.

    Ethical approval and consent to participate

    Not applicable.

    Consent for publication

    Not applicable.

    Copyright

    © The Author(s) 2022.

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    Cite This Article

    Sun Y, Wang J. How microplastics and nanoplastics shape antibiotic resistance?. Water Emerg Contam Nanoplastics 2022;1:8. http://dx.doi.org/10.20517/wecn.2022.09

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