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Figure 1. Lysosomal morphology and quantification in FD iPSC-podocytes. (A) Transmission Electron Microscopy (TEM) image of control iPSC-podocytes at day 10 of differentiation, showing rounded and electron-dense lysosomes in the cytosol (indicated by arrows). (B) TEM image of FD podocytes, showing enlarged and irregularly shaped lysosomes (arrows) and (C) myelin bodies (arrows), characteristic of FD. (D) FD podocytes show a significantly greater lysosomal area per field compared to controls on day 10 (**P < 0.01), which (E) correlated with a significant total lysosomal area (****P < 0.0001). Representative quantitative analysis of lysosomal area in FD and control podocytes, providing further validation of lysosomal dysregulation in FD. Data are presented as mean ± SEM.