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Figure 2. Immunostaining of treatment-naïve canine gliomas. (A, C, E) Representative immunostaining for (A) Iba1, (C) CD3, and (E) CD20 (40× magnification). (B, D, F) Numbers of (B) Iba1⁺ cells, (D) CD3⁺ lymphocytes, and (F) CD20⁺ lymphocytes within control (n = 3), astrocytoma (n = 9) and oligodendroglioma (n = 10) specimens. Using ordinary one-way ANOVA (column analyses) with Sidak’s multiple comparison test, statistical significance was calculated to correct the false discovery rate. Statistically significant differences were not noted despite obvious trends based on mean values for Astro and Oligo.