fig4

Figure 4. Representative 2-dimensional ddPCR plots of cardiovascular disease related microRNAs. ddPCR plots are separated into 4 quadrants representing FAM(-), VIC(-) events (lower left), FAM(-), VIC(+) events (lower right), FAM(+), VIC(-) events (upper left), and FAM(+) VIC(+) events (upper right). The analysis allows for quantification of total copy numbers, as well as a ratio of the microRNA of interest specific events [FAM(+), VIC(+)] normalized to the spike-in of miR39, represented by FAM(-), VIC(+) events.