fig5

Figure 5. Hair during the second cycle and root view of implanted scalp hair follicles. During the second hair cycle, regrown hair is shown before clipping (uncut panels on the left; D0 and D58), and after clipping (panels on the right: D58 and D60). The scale bar is shown at the top and bottom of the figure (spacing: 500 µm). Hair length increases during the D0-D58 interval, but the tips of non-dyed hair vs. dyed hair (D0 vs. D58) complicate precise LHGR. However, the combination of hair dye, clipping, and repeated imaging 48 h later (on D58 and D60; panels on the right) facilitates precise growth measurement. The latter is highlighted by the short blue segment in front of the arrowhead (panel D60) and the increased length is approximately 250 µm over 48 h, corresponding to a daily growth rate of 125 µm. Direct visualization of the anagen root, with its natural pigmentation, and the ghost image of the dermal papilla are marked with 11 red dots and align with our experimental design [schematic in Figure 1; green bar in Supplementary File 1]. D: Day; LHGR: linear hair growth rate measurements.