fig2

Figure 2. Extracellular vesicles isolated from osteoclasts, osteoclast precursors and 4T1 cancer cells have similar sizes and morphology. Top panels, (A) low magnification view of osteoclasts stained for tartrate-resistant acid phosphatase (TRAP) activity shows mostly multinuclear giant cells that stain pink indicating they are positive for TRAP; (B) Higher magnification view of osteoclast precursors, also stained for TRAP activity, shows no pink stain, only gold background staining; (C) Phalloidin-tagged with Texas Red demonstrates the 4T1 cancer cells; 4T1 cells do not contain TRAP. Scale bar in top panels is 200 µm for osteoclasts, and 10 µm for osteoclast precursors and 4T1 cells; Bottom panels show EVs isolated from (D) osteoclasts; (E) pre-osteoclasts and (F) 4T1 cells. Note that the EVs from the different cell types share similar size and morphology. Scale bars for bottom panels = 60 nm.